IL23 antibody

Catalog No. ABIN6964023

The Mouse Monoclonal anti-IL23 antibody has been validated for Neut. It is suitable to detect IL23 in samples from Human.

Quick Overview for IL23 antibody (ABIN6964023)

Target: IL23 (Interleukin 23 (IL23))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL23 antibody is un-conjugated

Application: Neutralization (Neut)

Clone: MT155

Product Details anti-IL23 Antibody

Specificity: Native and recombinant IL-23. The antibody recognizes the p19 subunit of the IL-23 heterodimer (p40+p19).

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Recombinant human IL-23

Isotype: IgG2b

Application Details

Application Notes: Neutralization of human IL-23 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. The antibody is also suitable for immunoassays. Neutralization Neutralization assay: Neutralization of human IL-23 induced IFN-γ production (see graph). To determine the neutralizing capacity and ND50, mixtures of various concentrations of mAb MT155, human recombinant IL-23 (10 ng/mL) and recombinant human IL-18 (50 ng/mL) were added to human PBMC. Following overnight incubation, cell supernatants were quantified in IFN-γ ELISA. Neutralization dose 50 (ND50): MT155 neutralized the effect of IL-23 at a ND50value of 0.2-0.8 μg/mL when 10 ng/mL recombinant human IL-23 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS free of preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Expiry Date: 18 months

Target Details for IL23

Target: IL23 (Interleukin 23 (IL23))

Alternative Name: IL23

Gene ID: 51561